MCRI Cell Culture Core Lab

The MCRI Cell Culture Core Laboratory is responsible for maintaining and growing the large biobank of human, murine, and other cell lines utilized to make scientific discoveries about how the cardiovascular system works at the cellular level. Functions of the Cell Culture Core Laboratory include isolation, maintenance and cryopreservation of cells, media preparation, training of investigators in aseptic and cell culture techniques, and consultation on novel cell culture methods. Cell proliferation assays, immortalization of cells, expansion of cell lines, establishment of transient and stable cloned cell lines, and preparation of cells for experiments are also provided. The core has the ability to establish new primary cell lines from a variety of tissues, especially vascular smooth muscle cells (SMC) and endothelial cells (EC) from humans, mice and other species.